NEFH antibody (CF®640R)
-
- Target See all NEFH Antibodies
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
-
Reactivity
- Human, Rat, Mouse, Chicken, Pig
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This NEFH antibody is conjugated to CF®640R
-
Application
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Neurofilament (NF-H) (RT-97), CF640R Conjugate
- Characteristics
- This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Triton-X 100 insoluble protein fraction of rat brain
- Clone
- RT-97
- Isotype
- IgG1
- Top Product
- Discover our top product NEFH Primary Antibody
-
-
- Application Notes
-
Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
-
Brain, Neuroblastoma
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
-
- Target
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- Alternative Name
- Neurofilament (NF-H) (NEFH Products)
- Synonyms
- NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
- Background
- NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200
- Molecular Weight
- 200 kDa
- Gene ID
- 4744, 198760
- UniProt
- P12036
-