Filaggrin antibody (AA 998-1104) (CF®488A)

Catalog No. ABIN6179754

Product Details anti-Filaggrin Antibody (CF®488A)

Target: Filaggrin (FLG)

Binding Specificity: AA 998-1104

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is conjugated to CF®488A

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt)

Purpose: Mouse Monoclonal anti-Filaggrin (FLG/1562), CF488A Conjugate

Characteristics: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Filaggrin protein fragment (aa 998-1104) (exact sequence is proprietary)

Clone: FLG-1562

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

U-251-MG cells or Skin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: Filaggrin (FLG Products)

Synonyms: AW107830 antibody, ft antibody, FLG antibody, ATOD2 antibody, Pflg antibody, Flg antibody, filaggrin antibody, Flg antibody, FLG antibody, LOC100351762 antibody, LOC100728006 antibody

Background: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin

Molecular Weight: 26-45 kDa (Processed), 350 kDa (Profilaggrin)

Gene ID: 2312, 654510

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance