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Myosin (Smooth Muscle Heavy Chain) antibody (CF®594)

Reactivity: Human, Rat, Chicken, Rabbit, Dog, Guinea Pig, Pig, Cat, Cow FACS, IF, IHC (f) Host: Mouse Monoclonal ID8 CF®594
Catalog No. ABIN6180022
  • Target See all Myosin (Smooth Muscle Heavy Chain) products
    Myosin (Smooth Muscle Heavy Chain)
    Reactivity
    Human, Rat, Chicken, Rabbit, Dog, Guinea Pig, Pig, Cat, Cow
    Host
    • 18
    Mouse
    Clonality
    • 18
    Monoclonal
    Conjugate
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Myosin (Smooth Muscle Heavy Chain) antibody is conjugated to CF®594
    Application
    • 17
    • 17
    • 17
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-Myosin, Smooth Muscle Heavy Chain (ID8), CF594 Conjugate
    Characteristics
    Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Human uterus extract
    Clone
    ID8
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Uterus or normal breast

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Myosin (Smooth Muscle Heavy Chain)
    Alternative Name
    Myosin, Smooth Muscle Heavy Chain (Myosin (Smooth Muscle Heavy Chain) Products)
    Background
    AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC
    Molecular Weight
    205 kDa (MHC-1) and 200 kDa (MHC-2)
    Gene ID
    4629, 460109
    UniProt
    P35749
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