CD33 antibody (CF®647)

Catalog No. ABIN6181337

Product Details anti-CD33 Antibody (CF®647)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Mouse Monoclonal anti-CD33 (C33/69), CF647 Conjugate

Characteristics: This antibody recognizes a 67 kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD33 protein

Clone: C33-69

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mLFlow Cytometry 0.5-1 μg/million cells/0.1 mL

Comment:

Human dendritic and mast cells. Human lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD33

Target: CD33

Alternative Name: CD33 (CD33 Products)

Synonyms: SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody

Background: Gp67, My9, Myeloid cell surface antigen CD33, p67, Sialic acid-binding Ig-like lectin 3 (SIGLEC3)

Molecular Weight: 67 kDa

Gene ID: 945, 83731

UniProt: P20138