CDX2 antibody (AA 150-249) (CF®647)

Catalog No. ABIN6182857

Product Details anti-CDX2 Antibody (CF®647)

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Binding Specificity: AA 150-249

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDX2 antibody is conjugated to CF®647

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Purpose: Mouse Monoclonal anti-CDX2 (CDX2/1690), CF647 Conjugate

Characteristics: The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CDX2 protein fragment (aa150-249) (exact sequence is proprietary)

Clone: CDX2-1690

Isotype: IgG2a kappa

Application Details

Application Notes: ELISA 2-4 μg/mL for coating (order Ab without BSA)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HT29 cells or Colon Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Alternative Name: CDX2 (CDX2 Products)

Synonyms: CDX2 antibody, MGC130987 antibody, cdx3-like antibody, CDX-3 antibody, CDX3 antibody, CDX-2 antibody, Cdx-2 antibody, caudal type homeobox 2 antibody, caudal type homeobox 2 L homeolog antibody, caudal type homeo box 2 antibody, CDX2 antibody, cdx2.L antibody, Cdx2 antibody

Background: Caudal type homeobox 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2, CDX2

Molecular Weight: 40 kDa

Gene ID: 1045, 174249

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance