CDC20 antibody (CF®568)
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- Target See all CDC20 Antibodies
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CDC20 antibody is conjugated to CF®568
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Cdc20 (AR12), CF568 Conjugate
- Characteristics
- Cyclins, regulatory subunits that associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Urea-denatured His6 Cdc20 human recombinant protein
- Clone
- AR12
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDC20 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Ramos or HeLa cells. Tonsil or gastric carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- Alternative Name
- Cdc20 (CDC20 Products)
- Synonyms
- X-FZY antibody, ba276h19.3 antibody, cdc20a antibody, fizzy1 antibody, p55cdc antibody, CDC20A antibody, bA276H19.3 antibody, p55CDC antibody, 2310042N09Rik antibody, C87100 antibody, cell division cycle 20 antibody, cell division cycle 20 L homeolog antibody, cell division cycle 20 homolog (S. cerevisiae) antibody, cdc20 antibody, CDC20 antibody, cdc20.L antibody, Cdc20 antibody
- Background
- CDC20, CDC20A, p55CDC, P55CDC-LSB
- Molecular Weight
- 55 kDa
- Gene ID
- 991, 524947
- UniProt
- Q12834
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