CD34 antibody (Biotin)

Catalog No. ABIN6185766

Product Details anti-CD34 Antibody (Biotin)

Target: CD34

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-CD34 (HPCA1/1171), Biotin Conjugate

Characteristics: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but shows low specificity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human HPCA1 protein

Clone: HPCA1-1171

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

KG-1 cells, Tonsil, or Angiosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Molecular Weight: 90-110 kDa

Gene ID: 947, 374990

UniProt: P28906