FBXO5 antibody (AA 1-250)
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- Target See all FBXO5 Antibodies
- FBXO5 (F-Box Protein 5 (FBXO5))
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Binding Specificity
- AA 1-250
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FBXO5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-EMI1 (EMI1/1176), Purified, BSA-free
- Characteristics
- This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
- Clone
- EMI1-1176
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product FBXO5 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Western blotting 1-2 μg/mL
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa, 293 or HepG2 cells. Ovarian carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Preservative
- Azide free
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- Target
- FBXO5 (F-Box Protein 5 (FBXO5))
- Alternative Name
- EMI1 (FBXO5 Products)
- Synonyms
- EMI1 antibody, FBX5 antibody, Fbxo31 antibody, 2510044I10Rik antibody, C85305 antibody, Emi1 antibody, emi1 antibody, fc65h02 antibody, wu:fc65h02 antibody, wu:fe06e07 antibody, wu:fz79f03 antibody, zgc:136397 antibody, zgc:158541 antibody, fbx5 antibody, Fbxo5 antibody, ACYPI007854 antibody, fbxo5 antibody, F-box protein 5 antibody, F-box only protein 5 antibody, F-box protein 5 L homeolog antibody, F-box protein 5 S homeolog antibody, FBXO5 antibody, Fbxo5 antibody, fbxo5 antibody, fbxo5.L antibody, fbxo5.S antibody
- Background
- Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31
- Molecular Weight
- 56 kDa
- Gene ID
- 26271, 520506
- UniProt
- Q9UKT4
- Pathways
- Mitotic G1-G1/S Phases
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