GP2 antibody (AA 35-179) (CF®640R)

Catalog No. ABIN6187109

Product Details anti-GP2 Antibody (CF®640R)

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Binding Specificity: AA 35-179

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GP2 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt)

Purpose: Mouse Monoclonal anti-GP2 / ZAP75 (GP2/1712), CF640R Conjugate

Characteristics: GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GP2 protein fragment (aa35-179) (exact sequence is proprietary)

Clone: GP2-1712

Isotype: IgG

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

PANC-1 cells. Pancreas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GP2

Target: GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))

Alternative Name: GP2 / ZAP75 (GP2 Products)

Synonyms: ZAP75 antibody, 2310037I18Rik antibody, AV060639 antibody, gp80 antibody, glycoprotein 2 antibody, glycoprotein 2 (zymogen granule membrane) antibody, GP2 antibody, Gp2 antibody

Target Type: Viral Protein

Background: Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75

Molecular Weight: 59 kDa

Gene ID: 2813, 53985

UniProt: P55259