CD21 antibody (CF®488A)
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- Target See all CD21 (CR2) Antibodies
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD21 antibody is conjugated to CF®488A
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Mouse Monoclonal anti-CD21 (FR5A10), CF488A Conjugate
- Characteristics
- This antibody recognizes a protein of 140 kDa, which is identified as the complement receptor 2 (CR2)/CD21. Its epitope is located in 5-8 short consensus repeats (SCRs). This MAb is highly specific to CR2 and shows no cross-reaction with CR1. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. MAb FR5A10 can be used for EBV receptor studies, interactions between B and T cells especially through CD23, human complement receptor (CR2) studies and IFN-alpha receptor studies. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Raji cells
- Clone
- FR5A10
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CR2 Primary Antibody
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- Application Notes
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Immunofluorescence 1-2 μg/mL, Immunohistology frozen 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))
- Alternative Name
- CD21 (CR2 Products)
- Synonyms
- C3DR antibody, CD21 antibody, CR antibody, CVID7 antibody, SLEB9 antibody, Cd21 antibody, cd21 antibody, CR1 antibody, CR1-L antibody, CR2 antibody, complement C3d receptor 2 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, CR2 antibody, Cr2 antibody, CR1 antibody
- Background
- CD21, Complement C3d receptor (C3DR), Complement Receptor type 2 (CR2), CVID7, EBV receptor, EBV-R, Epstein-Barr virus receptor, EVBR, SLEB9
- Molecular Weight
- 140 kDa
- Gene ID
- 1380, 445757
- UniProt
- P20023
- Pathways
- Complement System
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