MYB antibody (AA 119-135) (CF®488A)

Catalog No. ABIN6188381

Product Details anti-MYB Antibody (CF®488A)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Binding Specificity: AA 119-135

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYB antibody is conjugated to CF®488A

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-c-Myb (MYB286), CF488A Conjugate

Characteristics: The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-terminal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-myb oncoprotein, coupled to KLH

Clone: MYB286

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Jurkat, MOLT-4, COLO or BT-20 cells. Colon or breast carcinomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: c-Myb (MYB Products)

Synonyms: Cmyb antibody, c-myb antibody, c-myb_CDS antibody, efg antibody, AI550390 antibody, M16449 antibody, cmyb antibody, xmyb antibody, MYB proto-oncogene, transcription factor antibody, myeloblastosis oncogene antibody, MYB proto-oncogene, transcription factor S homeolog antibody, v-myb avian myeloblastosis viral oncogene homolog antibody, MYB antibody, Myb antibody, myb.S antibody, myb antibody

Background: C-myb_CDS, efg, ONCOGENE AMV, Oncogene MYB, Proto-oncogene c-Myb, Transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog

Molecular Weight: 75 kDa

Gene ID: 4602, 606320

UniProt: P01104

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process