MYB antibody (AA 119-135) (CF®594)
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- Target See all MYB Antibodies
- MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
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Binding Specificity
- AA 119-135
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MYB antibody is conjugated to CF®594
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Mouse Monoclonal anti-c-Myb (MYB286), CF594 Conjugate
- Characteristics
- The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-terminal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-myb oncoprotein, coupled to KLH
- Clone
- MYB286
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MYB Primary Antibody
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- Application Notes
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Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Jurkat, MOLT-4, COLO or BT-20 cells. Colon or breast carcinomas.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
- Alternative Name
- c-Myb (MYB Products)
- Synonyms
- Cmyb antibody, c-myb antibody, c-myb_CDS antibody, efg antibody, AI550390 antibody, M16449 antibody, cmyb antibody, xmyb antibody, MYB proto-oncogene, transcription factor antibody, myeloblastosis oncogene antibody, MYB proto-oncogene, transcription factor S homeolog antibody, v-myb avian myeloblastosis viral oncogene homolog antibody, MYB antibody, Myb antibody, myb.S antibody, myb antibody
- Background
- C-myb_CDS, efg, ONCOGENE AMV, Oncogene MYB, Proto-oncogene c-Myb, Transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog
- Molecular Weight
- 75 kDa
- Gene ID
- 4602, 606320
- UniProt
- P01104
- Pathways
- Chromatin Binding, Positive Regulation of Immune Effector Process
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