CHEK1 antibody (C-Term)
Quick Overview for CHEK1 antibody (C-Term) (ABIN6243849)
Target
See all CHEK1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 425-455, C-Term
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Predicted Reactivity
- C, Rat, X
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Purification
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Immunogen
- This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-455 amino acids from the C-terminal region of human CHK1.
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Isotype
- Ig Fraction
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anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibodyCHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibodyCHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibodyCHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibodyCHEK1 Reactivity: Human WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibodyCHEK1 Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 Reactivity: Human WB, IHC (p), DB Host: Rabbit Polyclonal RB7845-RB18425 unconjugated
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 Reactivity: Human, Mouse WB, ELISA Host: Mouse Monoclonal 2G1D5 unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 Reactivity: Mouse, Chicken ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody
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Application Notes
- WB: 1:2000
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Expiry Date
- 6 months
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- CHEK1 (Checkpoint Kinase 1 (CHEK1))
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Alternative Name
- CHK1
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Background
- Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.
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Molecular Weight
- 54434
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NCBI Accession
- NP_001107593, NP_001107594, NP_001231775, NP_001265
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UniProt
- O14757
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Pathways
- p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair
Target
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