Raptor antibody (AA 1005-1329)

Catalog No. ABIN6243898

Product Details anti-Raptor Antibody

Target: Raptor (RPTOR) (Regulatory Associated Protein of mTOR, Complex 1 (RPTOR))

Binding Specificity: AA 1005-1329

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Raptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This RPTOR antibody is generated from a mouse immunized with recombinant protein.

Clone: 1411CT316-2-151-34

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:500-1:1000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for Raptor

Target: Raptor (RPTOR) (Regulatory Associated Protein of mTOR, Complex 1 (RPTOR))

Alternative Name: RPTOR (RPTOR Products)

Synonyms: KOG1 antibody, Mip1 antibody, 4932417H02Rik antibody, Rap antibody, Raptor antibody, mKIAA1303 antibody, RGD1311784 antibody, raptor antibody, regulatory associated protein of MTOR complex 1 antibody, regulatory associated protein of MTOR, complex 1 antibody, regulatory associated protein of MTOR, complex 1 L homeolog antibody, RPTOR antibody, Rptor antibody, rptor.L antibody, rptor antibody

Background: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals, functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Molecular Weight: 149038

UniProt: Q8N122

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Autophagy, BCR Signaling, Warburg Effect