Multi Cytokeratin 4/5/6/8/10/13/18 antibody

Catalog No. ABIN6252020

Product Details

Target: Multi Cytokeratin 4/5/6/8/10/13/18

Reactivity: Marmoset, Mouse, Pig, Cow, Rat, Frog, Goat, Guinea Pig, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Protein G affinity chromatography

Immunogen: A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.

Clone: C11

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: Multi Cytokeratin 4/5/6/8/10/13/18

Background: This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.

Gene ID: 3851, 3852, 3853