Moesin antibody

Catalog No. ABIN6252085

Product Details anti-Moesin Antibody

Target: Moesin (MSN)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for this Moesin antibody.

Clone: SPM562

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the Moesin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: Moesin (MSN Products)

Synonyms: CG10701 antibody, D17 antibody, Dmel\\CG10701 antibody, Dmoe antibody, EMR1 antibody, ERM antibody, Emr1 antibody, anon-WO03040301.155 antibody, anon-WO03040301.157 antibody, anon-WO03040301.159 antibody, anon-WO03040301.161 antibody, dMoe antibody, gma antibody, l(1)G0067 antibody, l(1)G0323 antibody, l(1)G0404 antibody, l(1)G0415 antibody, moe antibody, moesin/ezrin/radixin homolog mRNA antibody, cb567 antibody, rdx antibody, wu:fb14b03 antibody, zgc:85640 antibody, MSN antibody, C78546 antibody, moesin antibody, Moesin antibody, moesin a antibody, moesin L homeolog antibody, MSN antibody, Moe antibody, msna antibody, msn.L antibody, moes antibody, Msn antibody

Background: Recognizes 78  kDa moesin protein. Moesin, a member of the Talin-4.1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

Gene ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization