NAPSA antibody
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- Target See all NAPSA Antibodies
- NAPSA (Napsin A Aspartic Peptidase (NAPSA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NAPSA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.
- Clone
- NAPSA-1239
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product NAPSA Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Napsin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- NAPSA (Napsin A Aspartic Peptidase (NAPSA))
- Alternative Name
- Napsin A (NAPSA Products)
- Synonyms
- KAP antibody, Kdap antibody, NAP1 antibody, NAPA antibody, SNAPA antibody, pronapsin antibody, kap antibody, kdap antibody, nap1 antibody, napa antibody, napsa antibody, snapa antibody, napsin A aspartic peptidase antibody, napsin A aspartic peptidase L homeolog antibody, NAPSA antibody, Napsa antibody, napsa.L antibody
- Background
- Napsin-A is a protein that in humans is encoded by the NAPSA gene. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [Wiki]
- Gene ID
- 9476
- UniProt
- O96009
- Pathways
- Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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