Transgelin antibody

Catalog No. ABIN6252141

Product Details anti-Transgelin Antibody

Target: Transgelin (TAGLN)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the SM22 alpha antibody. (3)

Clone: SMP22a

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the SM22 alpha antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. Cross-reaction with SM22 beta protein has not been determined.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: SM22 alpha (TAGLN Products)

Synonyms: MGC68550 antibody, sm22 antibody, smcc antibody, tagln1 antibody, ws3-10 antibody, MGC107855 antibody, SM22 antibody, SMCC antibody, TAGLN1 antibody, WS3-10 antibody, Sm22 antibody, Sm22a antibody, Ws310 antibody, SM22A antibody, zgc:153186 antibody, transgelin L homeolog antibody, transgelin antibody, tagln.L antibody, tagln antibody, LOC732906 antibody, TAGLN antibody, tagl antibody, LOC100009332 antibody, Tagln antibody

Background: SM22/Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart.

Gene ID: 6876

UniProt: Q01995