TNFRSF8 antibody

Catalog No. ABIN6252191

The Mouse Monoclonal anti-TNFRSF8 antibody (Clone CD30-412) (ABIN6252191) specifically detects TNFRSF8 in FACS, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for TNFRSF8 antibody (ABIN6252191)

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Clone: CD30-412

Product Details anti-TNFRSF8 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human CD30 recombinant protein was used as the immungen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: ELISA: order BSA-free format for coating

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD30 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TNFRSF8

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Alternative Name: CD30

Background: The antibody recognizes a single chain glycoprotein of 105/120  kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90  kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120  kDa glycoprotein. In Hodgkin's disease, CD30 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This antibody distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the CD30 positive lymphomas lack the leukocyte common antigen (CD45).

Gene ID: 943

UniProt: P28908