Keratin 6 antibody

Catalog No. ABIN6252256

Product Details anti-Keratin 6 Antibody

Target: Keratin 6 (KRT6)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant human CK6 protein was used as the immunogen for the Cytokeratin 6 antibody.

Clone: KRT6-1702

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT

Optimal dilution of the Cytokeratin 6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Keratin 6

Target: Keratin 6 (KRT6)

Alternative Name: Cytokeratin 6 (KRT6 Products)

Synonyms: Krt2-6 antibody, Krt-2.6 antibody, keratin 6 antibody, Krt6 antibody

Background: This mAb recognizes a protein of 56  kDa, identified as Cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Gene ID: 3853

UniProt: P02538