Keratin 6 antibody (C-Term)

Catalog No. ABIN6252257

Product Details anti-Keratin 6 Antibody

Target: Keratin 6 (KRT6)

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Protein G purified monoclonal antibody

Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from the C-terminus of human Cytokeratin 6 was used as the immunogen for this antibody.

Clone: LHK6

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl,  pH 2.0 for 10-15 min at RT.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Keratin 6

Target: Keratin 6 (KRT6)

Alternative Name: Cytokeratin 6 (KRT6 Products)

Synonyms: Krt2-6 antibody, Krt-2.6 antibody, keratin 6 antibody, Krt6 antibody

Background: This antibody recognizes a protein of 56  kDa, identified as cytokeratin 6 (CK6) or Keratin 6. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express Cytokeratin 6 and 16. Keratin 6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of Cytokeratin 6 is particularly associated with differentiation.

Gene ID: 3853

UniProt: P02538