Myogenin antibody (AA 138-158)
-
- Target See all Myogenin (MYOG) Antibodies
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
-
Binding Specificity
- AA 138-158
-
Reactivity
- Human, Mouse, Rat, Pig, Cat, Dog
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Myogenin antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
- Clone
- F5D
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MYOG Primary Antibody
-
-
- Application Notes
-
ELISA: order BSA-free format
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
- Target
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- Alternative Name
- Myogenin (MYOG Products)
- Synonyms
- cb553 antibody, zgc:100763 antibody, LOC446037 antibody, myf4 antibody, Xmyog antibody, myogenin antibody, LOC100136471 antibody, LOC100224062 antibody, LOC100303673 antibody, MYF4 antibody, bHLHc3 antibody, myo antibody, myf-4 antibody, myogenin antibody, myogenin (myogenic factor 4) antibody, MYOG antibody, myog antibody, LOC100303673 antibody, Myog antibody
- Background
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
- Gene ID
- 4656
- UniProt
- P15173
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
-