KRT15 antibody (C-Term)

Catalog No. ABIN6252303

Product Details anti-KRT15 Antibody

Target: KRT15 (Keratin 15 (KRT15))

Binding Specificity: C-Term

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A 17 amino acid sequence from the C- terminal of human CK15 was used as the immunogen for the Keratin 15 antibody.

Clone: LHK15

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

Immunohistochemistry (FFPE & Frozen): 0.5-1 μg/mL for 30 min at RT

Optimal dilution of the Keratin 15 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: Cytokeratin 15 (KRT15 Products)

Synonyms: xlk2 antibody, CK15 antibody, K15 antibody, K1CO antibody, AI528832 antibody, Krt1-15 antibody, Ka15 antibody, wu:fa91c06 antibody, wu:fa95d04 antibody, zgc:55334 antibody, keratin 15, type I L homeolog antibody, keratin 15, type I antibody, keratin 15 antibody, krt15.L antibody, krt15 antibody, KRT15 antibody, Krt15 antibody

Background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Gene ID: 3866

UniProt: P19012