CD79a antibody (AA 202-216)
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- Target See all CD79a (CD79A) Antibodies
- CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))
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Binding Specificity
- AA 202-216
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD79a antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for this CD79a antibody.
- Clone
- JCB117
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD79A Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD79a antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))
- Alternative Name
- CD79a (CD79A Products)
- Synonyms
- CD79A antibody, Ig-alpha antibody, Iga antibody, Igalpha antibody, Ly-54 antibody, Ly54 antibody, mb-1 antibody, IGA antibody, MB-1 antibody, IG-alpha antibody, CD79a molecule antibody, CD79A antigen (immunoglobulin-associated alpha) antibody, Cd79a molecule-like antibody, CD79A antibody, Cd79a antibody, Cd79al antibody
- Background
- A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B-cell Ag receptor. CD79a first appears at pre B-cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. It is found in the majority of acute leukemias of B-cell precursors, lines and lymphomas, and in some myelomas. It is not present in myeloid or Tcell lines. CD79a antibody is generally used to complement CD20 mAb, especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This CD79a antibody will stain many of the same lymphomas as CD20 mAb, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 mAb. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
- Gene ID
- 973
- UniProt
- P11912
- Pathways
- BCR Signaling
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