GRP94 antibody

Catalog No. ABIN6252499

Product Details anti-GRP94 Antibody

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the GRP94 antibody.

Clone: HSP90B1-1192

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistology (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the GRP94 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: GRP94 (HSP90B1 Products)

Synonyms: ecgp antibody, gp96 antibody, tra1 antibody, grp94 antibody, DDBDRAFT_0206450 antibody, DDBDRAFT_0215015 antibody, DDB_0206450 antibody, DDB_0215015 antibody, TRA1 antibody, HSP90B1 antibody, ERp99 antibody, GRP94 antibody, TA-3 antibody, Targ2 antibody, Tra-1 antibody, Tra1 antibody, endoplasmin antibody, GP96 antibody, fb61d09 antibody, tra-1 antibody, wu:fb61d09 antibody, wu:fq25g01 antibody, Grp94 antibody, ECGP antibody, Gp96 antibody, ppk98 antibody, hsp108 antibody, heat shock protein 90kDa beta (Grp94), member 1 antibody, heat shock protein Hsp90 family protein antibody, 94 kD glucose-regulated protein antibody, heat shock protein 90 beta family member 1 antibody, heat shock protein 90, beta (Grp94), member 1 antibody, heat shock protein 90, beta (grp94), member 1 antibody, heat shock protein 90kDa beta family member 1 L homeolog antibody, hsp90b1 antibody, grp94 antibody, HSP90B1 antibody, Hsp90b1 antibody, hsp90b1.L antibody

Background: Recognizes a protein of 94  kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Gene ID: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades