CA9 antibody

Catalog No. ABIN6252592

Product Details anti-CA9 Antibody

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogen: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.

Clone: 66-4-C2

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CAIX antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CAIX (CA9 Products)

Synonyms: CA9 antibody, CAIX antibody, Ca9 antibody, MN/CA9 antibody, MN antibody, carbonic anhydrase 9 antibody, CA9 antibody, CAH9 antibody, Car9 antibody

Background: This antibody recognizes a glycoprotein of ~200  kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.

Gene ID: 768

UniProt: Q16790