MUC5AC antibody

Catalog No. ABIN6252704

Product Details anti-MUC5AC Antibody

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig, Hedgehog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: Does not react with cow

Purification: Protein G affinity chromatography

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.

Clone: 45M1

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: MUC5AC (MUC5AC Products)

Synonyms: MUC5 antibody, TBM antibody, leB antibody, 2210005L13Rik antibody, MGM antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, putative gastric mucin antibody, mucin 5, subtypes A and C, tracheobronchial/gastric antibody, mucin 2, oligomeric mucus/gel-forming antibody, MUC5AC antibody, Smp_144070 antibody, Muc5ac antibody, MUC2 antibody

Background: This antibody recognizes the peptide core of gastric mucin M1 (>1,000  kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Gene ID: 4586

UniProt: P98088