MUC3A antibody
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- Target See all MUC3A products
- MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MUC3A antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Does not react with rat
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.
- Clone
- MUCN3-1
- Isotype
- IgG2a kappa
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- Application Notes
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Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the MUC3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- MUC3A (Mucin 3A, Cell Surface Associated (MUC3A))
- Alternative Name
- MUC3 (MUC3A Products)
- Synonyms
- Muc3 antibody, MUC-3A antibody, MUC3 antibody, mucin 3, intestinal antibody, mucin 3A, cell surface associated antibody, Muc3 antibody, MUC3A antibody
- Background
- The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats. Mucin-3/MUC3 is a major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling. [UniProt]
- Gene ID
- 4584
- UniProt
- Q02505
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