pan Keratin antibody

Catalog No. ABIN6252865

Product Details anti-pan Keratin Antibody

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Frog, Pig, Guinea Pig, Goat, Marmoset

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Protein G affinity chromatography

Immunogen: A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.

Clone: SPM583

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Multi-Cytokeratin (panKRT Products)

Synonyms: xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody

Background: This mAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Gene ID: 3851, 3852, 3853

UniProt: P19013, P13647, P02538, P04259, P48668, P05787, P13645, P13646, P05783