HIF1A antibody (C-Term)

Catalog No. ABIN6254227

This Goat Polyclonal antibody specifically detects HIF1A in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Chicken, Dog, Rabbit, Pig, Monkey, Goat, Horse, Hamster, Guinea Pig, Sheep, Donkey, Cat and Avian. It has been mentioned in 4+ publications

Quick Overview for HIF1A antibody (C-Term) (ABIN6254227)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Rabbit, Pig, Monkey, Goat, Horse, Hamster, Guinea Pig, Sheep, Donkey, Cat, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HIF1A Antibody

Binding Specificity: C-Term

Purpose: Anti-HIF1a

Sequence: LLGQSMDESG LPQLTSYDCE VNAPIQGSRN LLQGEELLRA LDQVN

Specificity: Detects HIF1a in transfected cells and a band of approximately 120 kDa by Western blot in cell lysates.

Characteristics: Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 780 aa to the C-terminus of human HIF1a produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-HIF1A antibody (ABIN6254227)

Ferreira, Rosa Soares, Ramalho, Ribeiro-Rodrigues, Máximo, Zuzarte, Girão, Pereira: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).

Rodrigues, Matafome, Sereno, Almeida, Castelhano, Gamas, Neves, Gonçalves, Carvalho, Arslanagic, Wilcken, Fonseca, Simões, Conde, Castelo-Branco, Seiça: "Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance." in: Scientific reports, Vol. 7, Issue 1, pp. 1698, (2018) (PubMed).

Sacramento, Ribeiro, Rodrigues, Guarino, Diogo, Seiça, Monteiro, Matafome, Conde: "Insulin resistance is associated with tissue-specific regulation of HIF-1α and HIF-2α during mild chronic intermittent hypoxia." in: Respiratory physiology & neurobiology, Vol. 228, pp. 30-8, (2017) (PubMed).

Ferreira, Soares, Ramalho, Pereira, Girao: "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy." in: Scientific reports, Vol. 5, pp. 10210, (2016) (PubMed).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF1a

Background:

Goat polyclonal to alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in apoptosis, angiogenesis, energy metabolism, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in tumour angiogenesis, embryonic vascularization, and pathophysiology of ischemic disease.

alpha subunit (basic helix-loop-helix transcription factor), ARNT interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF-1alpha, HIF1, HIF1-ALPHA, HIF1a, hypoxia inducible factor 1, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, hypoxia-inducible factor 1-alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PASD8, PAS domain-containing protein 8 antibody.

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect