RHOA antibody (pSer188)

Catalog No. ABIN6256176

Product Details anti-RHOA Antibody

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Binding Specificity: pSer188

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Phospho-RhoA (Ser188) Antibody detects endogenous levels of RhoA only when phosphorylated at Serine 188

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human RhoA around the phosphorylation site of Serine 188

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RhoA (RHOA Products)

Synonyms: ARH12 antibody, ARHA antibody, RHO12 antibody, RHOH12 antibody, Arha antibody, Arha1 antibody, Arha2 antibody, arha antibody, arha2 antibody, xrhoa antibody, arh12 antibody, rho12 antibody, rhoh12 antibody, rhoa antibody, RHO1 antibody, Rhoa antibody, wu:fa66g04 antibody, wu:fb26g07 antibody, zgc:66058 antibody, zgc:85805 antibody, ARHC antibody, rhoae antibody, zgc:64015 antibody, ras homolog family member A antibody, ras homolog family member A S homeolog antibody, ras homolog family member A L homeolog antibody, ras homolog gene family, member Aa antibody, ras homolog family member Ca antibody, RHOA antibody, Rhoa antibody, rhoa.S antibody, rhoa antibody, rhoa.L antibody, rhoaa antibody, rhoca antibody

Background:

Description: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation, promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).

Gene: RHOA

Molecular Weight: 22kDa

Gene ID: 387

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome