AIM2 antibody (Internal Region)

Catalog No. ABIN6256925

This Rabbit Polyclonal antibody specifically detects AIM2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for AIM2 antibody (Internal Region) (ABIN6256925)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-AIM2 Antibody

Binding Specificity: Internal Region

Specificity: AIM2 Antibody detects endogenous levels of total AIM2.

Predicted Reactivity: Horse

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AIM2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: AIM2

Background:

Description: Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.

Gene: AIM2

Molecular Weight: 39 kDa

Gene ID: 9447

UniProt: O14862

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome