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AIM2 antibody (Absent in Melanoma 2) (AA 14-215) Primary Antibody

AIM2 Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3043780
$280.00
Plus shipping costs $45.00
100 μg
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  • Target
    AIM2
    Binding Specificity
    AA 14-215
    Reactivity
    Human, Mouse, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This AIM2 antibody is un-conjugated
    Application
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Interferon-inducible protein AIM2(AIM2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Interferon-inducible protein AIM2(AIM2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: absent in melanoma 2
    Protein Name: Interferon-inducible protein AIM2
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    AIM2
    Alternative Name
    AIM2 (AIM2 Antibody Abstract)
    Synonyms
    AIM2, PYHIN4, Gm1313, Ifi210, absent in melanoma 2, AIM2, Aim2
    Background
    Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is a protein that in humans is encoded by the AIM2 gene. It is mapped to 1q22. AIM2 is a member of the Ifi202/IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains a CARD domain (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines (IL-1b and IL-18).

    Synonyms: Absent in melanoma 2 antibody|AIM 2 antibody|Aim2 antibody|AIM2_HUMAN antibody|Interferon-inducible protein AIM2 antibody| OTTHUMP00000035296 antibody|PYHIN4 antibody
    Gene ID
    9447
    UniProt
    O14862
    Pathways
    Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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