BRSK2 antibody (Internal Region)

Catalog No. ABIN6260317

Product Details anti-BRSK2 Antibody

Target: BRSK2 (BR serine/threonine Kinase 2 (BRSK2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRSK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: BRSK2 Antibody detects endogenous levels of total BRSK2.

Predicted Reactivity: Pig,Bovine,Horse,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human BRSK2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for BRSK2

Target: BRSK2 (BR serine/threonine Kinase 2 (BRSK2))

Alternative Name: BRSK2 (BRSK2 Products)

Synonyms: C11orf7 antibody, PEN11B antibody, SAD1 antibody, SADA antibody, STK29 antibody, BRSK2 antibody, 4833424K13Rik antibody, SAD-A antibody, BR serine/threonine kinase 2 antibody, BRSK2 antibody, Brsk2 antibody

Background:

Description: Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.

Gene: BRSK2

Molecular Weight: 82 kDa

Gene ID: 9024

UniProt: Q8IWQ3