CHAC1 antibody (Internal Region)

Catalog No. ABIN6260788

Product Details anti-CHAC1 Antibody

Target: CHAC1 (ChaC, Cation Transport Regulator Homolog 1 (CHAC1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: CHAC1 Antibody detects endogenous levels of total CHAC1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CHAC1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CHAC1

Target: CHAC1 (ChaC, Cation Transport Regulator Homolog 1 (CHAC1))

Alternative Name: CHAC1 (CHAC1 Products)

Synonyms: 1810008K03Rik antibody, RGD1307153 antibody, ChaC glutathione specific gamma-glutamylcyclotransferase 1 antibody, ChaC, cation transport regulator 1 antibody, ChaC glutathione-specific gamma-glutamylcyclotransferase 1 antibody, CHAC1 antibody, Chac1 antibody

Background:

Description: Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides (PubMed:27913623). Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).

Gene: CHAC1

Molecular Weight: 29kDa

Gene ID: 79094

UniProt: Q9BUX1