MPHOSPH8 antibody (Internal Region)
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- Target See all MPHOSPH8 Antibodies
- MPHOSPH8 (M-Phase phosphoprotein 8 (MPHOSPH8))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MPHOSPH8 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- MPHOSPH8 Antibody detects endogenous levels of total MPHOSPH8.
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human MPHOSPH8, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product MPHOSPH8 Primary Antibody
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- Application Notes
- WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- MPHOSPH8 (M-Phase phosphoprotein 8 (MPHOSPH8))
- Alternative Name
- MPHOSPH8 (MPHOSPH8 Products)
- Synonyms
- HSMPP8 antibody, TWA3 antibody, mpp8 antibody, 1500035L22Rik antibody, 4930548G07Rik antibody, AU040635 antibody, Ab2-008 antibody, RGD1305133 antibody, M-phase phosphoprotein 8 antibody, MPHOSPH8 antibody, Mphosph8 antibody
- Background
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Description: Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites, these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).
Gene: MPHOSPH8
- Molecular Weight
- 97 kDa
- Gene ID
- 54737
- UniProt
- Q99549
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