NPC2 antibody (N-Term)

Catalog No. ABIN6263698

Product Details anti-NPC2 Antibody

Target: NPC2 (Niemann-Pick Disease, Type C2 (NPC2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPC2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: NPC2 Antibody detects endogenous levels of total NPC2.

Predicted Reactivity: Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NPC2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NPC2

Target: NPC2 (Niemann-Pick Disease, Type C2 (NPC2))

Alternative Name: NPC2 (NPC2 Products)

Synonyms: 2700012J19Rik antibody, AA408070 antibody, AU045843 antibody, HE1 antibody, EDDM1 antibody, re1 antibody, CE1 antibody, EPI-1 antibody, cb292 antibody, sb:cb292 antibody, NPC intracellular cholesterol transporter 2 antibody, Niemann-Pick disease, type C2 antibody, Npc2 antibody, NPC2 antibody, npc2 antibody

Background:

Description: Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.

Gene: NPC2

Molecular Weight: 17kDa

Gene ID: 10577

UniProt: P61916

Pathways: SARS-CoV-2 Protein Interactome