PRKRA antibody (Internal Region)

Catalog No. ABIN6264430

Product Details anti-PRKRA Antibody

Target: PRKRA (Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent Activator (PRKRA))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PRKRA Antibody detects endogenous levels of total PRKRA.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PRKRA, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PRKRA

Target: PRKRA (Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent Activator (PRKRA))

Alternative Name: PRKRA (PRKRA Products)

Synonyms: PRKRA antibody, AV120107 antibody, PRK antibody, Pact antibody, RAX antibody, lear antibody, DYT16 antibody, PACT antibody, im:7151758 antibody, zgc:162619 antibody, dyt16 antibody, pact antibody, prkra-a antibody, prkra-b antibody, rax antibody, rbpa antibody, protein activator of interferon induced protein kinase EIF2AK2 antibody, protein kinase, interferon inducible double stranded RNA dependent activator antibody, protein kinase, interferon-inducible double stranded RNA dependent activator antibody, protein activator of interferon induced protein kinase EIF2AK2 L homeolog antibody, PRKRA antibody, Prkra antibody, prkra antibody, prkra.L antibody

Background:

Description: Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).

Gene: PRKRA

Molecular Weight: 34kDa

Gene ID: 8575

UniProt: O75569

Pathways: Regulatory RNA Pathways