SH2D1B antibody (Internal Region)

Catalog No. ABIN6265050

Product Details anti-SH2D1B Antibody

Target: SH2D1B (SH2 Domain Containing 1B (SH2D1B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH2D1B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: SH2D1B Antibody detects endogenous levels of total SH2D1B.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SH2D1B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SH2D1B

Target: SH2D1B (SH2 Domain Containing 1B (SH2D1B))

Alternative Name: SH2D1B (SH2D1B Products)

Synonyms: EAT2 antibody, Sh2d1b1 antibody, RGD1563935 antibody, SH2 domain containing 1B antibody, SH2D1B antibody, Sh2d1b antibody

Background:

Description: Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation, downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (By similarity). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation (By similarity).

Gene: SH2D1B

Molecular Weight: 15 kDa

Gene ID: 117157

UniProt: O14796

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process