TPMT antibody (N-Term)

Catalog No. ABIN6265692

The Rabbit Polyclonal anti-TPMT antibody has been validated for WB, ELISA and IHC. It is suitable to detect TPMT in samples from Human, Mouse and Rat.

Quick Overview for TPMT antibody (N-Term) (ABIN6265692)

Target: TPMT (Thiopurine S-Methyltransferase (TPMT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TPMT Antibody

Binding Specificity: N-Term

Specificity: TPMT Antibody detects endogenous levels of total TPMT.

Predicted Reactivity: Bovine,Horse,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TPMT, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TPMT

Target: TPMT (Thiopurine S-Methyltransferase (TPMT))

Alternative Name: TPMT

Background:

Description: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor (PubMed:657528, PubMed:18484748). TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.

Gene: TPMT

Molecular Weight: 28kDa

Gene ID: 7172

UniProt: P51580