VPRBP antibody
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- Target See all VPRBP Antibodies
- VPRBP (Vpr (HIV-1) Binding Protein (VPRBP))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This VPRBP antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- VPRBP antibody detects endogenous levels of VPRBP.
- Cross-Reactivity
- Human, Mouse (Murine), Rat (Rattus)
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human VPRBP.
- Isotype
- IgG
- Top Product
- Discover our top product VPRBP Primary Antibody
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
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- Target
- VPRBP (Vpr (HIV-1) Binding Protein (VPRBP))
- Alternative Name
- VPRBP (VPRBP Products)
- Synonyms
- RGD1305176 antibody, DCAF1 antibody, AI447437 antibody, B930007L02Rik antibody, mKIAA0800 antibody, DDB1 and CUL4 associated factor 1 antibody, protein VPRBP antibody, Vpr (HIV-1) binding protein antibody, Dcaf1 antibody, DCAF1 antibody, LOC100175926 antibody, vprbp antibody, dcaf1 antibody
- Target Type
- Viral Protein
- Background
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Description: Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714).
Gene: DCAF1
- Molecular Weight
- 169 kDa
- Gene ID
- 9730
- UniProt
- Q9Y4B6
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