WT1 antibody (N-Term)

Catalog No. ABIN6276599

Product Details anti-WT1 Antibody

Target: WT1 (Wilms Tumor 1 (WT1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: WT1 Antibody detects endogenous levels of total WT1.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human WT1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 (WT1 Products)

Synonyms: AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody

Background:

Description: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Gene: WT1

Molecular Weight: 49kDa

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation