STS antibody (N-Term)

Catalog No. ABIN6280371

Product Details anti-STS Antibody

Target: STS (Steroid Sulfatase (STS))

Binding Specificity: AA 10-90, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: ASC is a protein encoded by the PYCARD gene which is approximately 21,6 kDa. ASC is localised to the cytoplasm, endoplasmic reticulum, mitochondrion and nucleus. It is involved in toll-like receptor signalling pathways, CLEC7A (Dectin-1) signalling and the innate immune system. It functions as key mediator in apoptosis and inflammation. It promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a cell type-specific manner. ASC is widely expressed at low levels in human tissues. Mutations in the PYCARD gene may result in chronic recurrent multifocal osteomyelitis and Cinca syndrome. STJ91730 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of ASC protein.

Specificity: ASC Polyclonal Antibody detects endogenous levels of ASC protein.

Characteristics: Rabbit polyclonal to ASC.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ASC

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000

Comment:

Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for STS

Target: STS (Steroid Sulfatase (STS))

Alternative Name: ASC (STS Products)

Synonyms: 9130417A21Rik antibody, Asc antibody, CARD5 antibody, TMS-1 antibody, TNS1 antibody, masc antibody, ARSC antibody, ARSC1 antibody, ASC antibody, ES antibody, SSDD antibody, XLI antibody, PYD and CARD domain containing antibody, steroid sulfatase antibody, Pycard antibody, STS antibody, Sts antibody

Molecular Weight: 21 kDa

Gene ID: 29108

UniProt: Q9ULZ3

Pathways: Steroid Hormone Biosynthesis, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation