STS antibody (N-Term)
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- Target See all STS Antibodies
- STS (Steroid Sulfatase (STS))
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Binding Specificity
- AA 10-90, N-Term
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This STS antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- ASC is a protein encoded by the PYCARD gene which is approximately 21,6 kDa. ASC is localised to the cytoplasm, endoplasmic reticulum, mitochondrion and nucleus. It is involved in toll-like receptor signalling pathways, CLEC7A (Dectin-1) signalling and the innate immune system. It functions as key mediator in apoptosis and inflammation. It promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a cell type-specific manner. ASC is widely expressed at low levels in human tissues. Mutations in the PYCARD gene may result in chronic recurrent multifocal osteomyelitis and Cinca syndrome. STJ91730 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of ASC protein.
- Specificity
- ASC Polyclonal Antibody detects endogenous levels of ASC protein.
- Characteristics
- Rabbit polyclonal to ASC.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human ASC
- Isotype
- IgG
- Top Product
- Discover our top product STS Primary Antibody
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- Application Notes
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WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000 - Comment
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Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- STS (Steroid Sulfatase (STS))
- Alternative Name
- ASC (STS Products)
- Synonyms
- 9130417A21Rik antibody, Asc antibody, CARD5 antibody, TMS-1 antibody, TNS1 antibody, masc antibody, ARSC antibody, ARSC1 antibody, ASC antibody, ES antibody, SSDD antibody, XLI antibody, PYD and CARD domain containing antibody, steroid sulfatase antibody, Pycard antibody, STS antibody, Sts antibody
- Molecular Weight
- 21 kDa
- Gene ID
- 29108
- UniProt
- Q9ULZ3
- Pathways
- Steroid Hormone Biosynthesis, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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