IKK-alpha /IKK-beta antibody (pSer176, pSer177)

Catalog No. ABIN6281085

Product Details anti-IKK-alpha /IKK-beta Antibody

Target: IKK-alpha /IKK-beta

Binding Specificity: AA 120-200, pSer176, pSer177

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK-alpha /IKK-beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: IKKα/β is a protein encoded by the CHUK gene which is approximately 84,6 kDa. IKKα/β is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the TNFR1 pathway, the 4-1BB pathway, RET signalling and toll-like receptor signalling pathways. This protein falls under the serine/threonine protein kinase family. It has an essential role in the NF-kappa-B signalling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. These modifications allow for degradation by the proteasome. IKKα/β is widely expressed. Mutations in the CHUK gene result in Cocoon syndrome and incontinentia pigmenti. STJ90301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IKKα/β protein which only binds about S176/177 when S176/177 is phosphorylated.

Specificity: Phospho-IKKα/β (S176/177) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S176/177.

Characteristics: Rabbit polyclonal to Phospho-IKKα/β (S176/177).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IKKalpha/beta around the phosphorylation site of S176/177.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Comment:

Widely expressed.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Alternative Name: IKKalpha/beta (IKK-alpha /IKK-beta Products)

Molecular Weight: 80 kDa

Gene ID: 1147

UniProt: O15111