ERK1/2 antibody (pThr202)

Catalog No. ABIN6281338

Product Details anti-ERK1/2 Antibody

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Binding Specificity: AA 140-220, pThr202

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: ERK 1/2 is a protein encoded by the MAPK1 gene which is approximately 41,4 kDa. ERK 1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, activated TLR4 signalling, IL-2 pathway, regulation of lipid metabolism and insulin signalling-generic cascades. This protein falls under the MAP kinase family. It is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. It also acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK 1/2 is expressed in the nervous system, blood, lung, liver and skin. Mutations in the MAPK1 gene may result in small intestine neuroendocrine neoplasm and pertussis. STJ90260 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous ERK 1/2 protein which only binds about T202 when T202 is phosphorylated.

Specificity: Phospho-ERK 1/2 (T202) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at T202.

Characteristics: Rabbit polyclonal to Phospho-ERK 1/2 (T202).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ERK 1/2 around the phosphorylation site of T202.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ELISA 1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: ERK 1/2 (MAPK1/3 Products)

Synonyms: erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody

Molecular Weight: 44/42 kDa

Gene ID: 5595

UniProt: P27361