MEK1/2 antibody (pSer218, pSer222)

Catalog No. ABIN6281619

Product Details anti-MEK1/2 Antibody

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Binding Specificity: AA 160-240, pSer218, pSer222

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: MEK-1/2 is a protein encoded by the MAP2K1 gene which is approximately 43,4 kDa. MEK-1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, activated TLR4 signalling, the IL-2 pathway, regulation of lipid metabolism and insulin signalling-generic cascades. It acts as an integration point for multiple biochemical signals such as proliferation, differentiation, transcription regulation and development. It is also a dual-specificity kinase known for its involvement in the ERK pathway by activation of ERK1 and ERK2. MEK-1/2 is widely expressed with low levels in the brain. Mutations in the MAP2K1 gene may result in Cardiofaciocutaneous syndrome. STJ90327 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MEK-1/2 protein which only binds about S218/222 when S218/222 is phosphorylated.

Specificity: Phospho-MEK-1/2 (S218/222) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S218/222.

Characteristics: Rabbit polyclonal to Phospho-MEK-1/2 (S218/222).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MEK-1/2 around the phosphorylation site of S218/222.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Comment:

Widely expressed, with extremely low levels in brain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Alternative Name: MEK-1/2 (MAP2K1/2 Products)

Molecular Weight: 48 kDa

Gene ID: 5604

UniProt: Q02750