Caspase-8 p18 antibody (Cleaved-Ser217, Internal Region)

Catalog No. ABIN6281720

Product Details anti-Caspase-8 p18 Antibody

Target: Caspase-8 p18 (Caspase 8 Subunit P18)

Binding Specificity: AA 170-250, Cleaved-Ser217, Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase-8 p18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Caspase-8 is a protein encoded by the CASP8 gene which is approximately 55,3 kDa. Caspase-8 is localised to the cytoplasm and is involved in the TNFR1 pathway, dimerization of procaspase-8 , activated TLR4 signalling, apoptosis signalling and toll-like receptor signalling pathways. This protein falls under the cysteine-aspartic acid protease family. It pays a role in the programmed cell death induced by Fas and various apoptotic stimuli. Caspase-8 isoform 1, 5 and 7 are expressed in a wide variety of tissues. Mutations in the CASP8 gene may result in a caspase-8 deficiency. STJ97641 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.This polyclonal antibody detects endogenous levels of fragment of activated caspase-8 p18 protein resulting from cleavage adjacent to S217.

Specificity: Cleaved-Caspase-8 p18 (S217) Polyclonal Antibody detects endogenous levels of Cleaved-Caspase-8 p18 (S217) protein.

Characteristics: Rabbit polyclonal to Cleaved-Caspase-8 p18 (S217).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cleaved-Caspase-8 p18 (S217)

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ELISA 1:10000

Comment:

Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Caspase-8 p18

Target: Caspase-8 p18 (Caspase 8 Subunit P18)

Alternative Name: Caspase-8 p18 (Caspase-8 p18 Products)

Synonyms: ALPS2B antibody, CAP4 antibody, Casp-8 antibody, FLICE antibody, MACH antibody, MCH5 antibody, CASP-8 antibody, Mch5 antibody, caspase 8 antibody, CASP8 antibody, Casp8 antibody

Gene ID: 841

UniProt: Q14790