PI3K p85 alpha/gamma antibody (AA 410-490)

Catalog No. ABIN6283949

Product Details anti-PI3K p85 alpha/gamma Antibody

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Binding Specificity: AA 410-490

Reactivity: Human, Mouse, Rat, Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3K p85 alpha/gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PI 3-kinase p85α/γ is a protein encoded by the PIK3R1 gene which is approximately 83,5 kDa. PI 3-kinase p85α/γ is localised to the cytoplasm, nucleus and cis-Golgi network. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2 and regulation of lipid metabolism. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter by mediating the association of the p110 catalytic unit to the plasma membrane. It is also necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85α/γ isoform 2 is expressed in skeletal muscle and brain. Mutations in the PIK3R1 gene may result in Short syndrome and Pasli disease. STJ95078 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PI 3-kinase p85α/γ protein.

Specificity: PI 3-kinase p85α/γ Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α/γ protein.

Characteristics: Rabbit polyclonal to PI 3-kinase p85α/γ.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Y458/199.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Comment:

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PI3K p85 alpha/gamma

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Alternative Name: PI 3-kinase p85alpha/gamma (PI3K p85a/g Products)

Molecular Weight: 83/54 kDa

Gene ID: 5295

UniProt: P27986