p21 antibody (AA 80-160)
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- Target See all p21 (CDKN1A) Antibodies
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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Binding Specificity
- AA 80-160
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This p21 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- p21 is a protein encoded by the CDKN1A gene which is approximately 18,1 kDa. p21 is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, cyclins and cell cycle regulation. It may be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. It binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. It also functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. p21 is expressed in all adult tissues, with lower levels expressed in the brain. Mutations in the CDKN1A gene may result in pancreatic cancer and ovarian cancer. STJ94862 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of p21 protein.
- Specificity
- P21 Polyclonal Antibody detects endogenous levels of p21 protein.
- Characteristics
- Rabbit polyclonal to p21.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human p21 around the non-phosphorylation site of T145.
- Isotype
- IgG
- Top Product
- Discover our top product CDKN1A Primary Antibody
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- Application Notes
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WB 1:500-1:2000
ELISA 1:20000 - Comment
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Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- Alternative Name
- p21 (CDKN1A Products)
- Synonyms
- CCND1 antibody, CDKN1A antibody, CAP20 antibody, CDKN1 antibody, CIP1 antibody, MDA-6 antibody, P21 antibody, SDI1 antibody, WAF1 antibody, p21CIP1 antibody, CDKI antibody, Cdkn1 antibody, Waf1 antibody, mda6 antibody, p21Cip1 antibody, p21WAF antibody, Cip1 antibody, UV96 antibody, p21 antibody, cip1 antibody, p16Xic2 antibody, sdi1 antibody, waf1 antibody, cap20 antibody, cdkn1 antibody, mda-6 antibody, p21cip1 antibody, si:ch1073-48m5.2 antibody, KRAS2 antibody, p21ras antibody, cyclin D1 antibody, cyclin dependent kinase inhibitor 1A antibody, cyclin-dependent kinase inhibitor 1A (P21) antibody, cyclin-dependent kinase inhibitor 1A antibody, cyclin-dependent kinase inhibitor 1A L homeolog antibody, cyclin-dependent kinase inhibitor 1 antibody, KRAS proto-oncogene, GTPase antibody, CCND1 antibody, CDKN1A antibody, Cdkn1a antibody, cdkn1a.L antibody, cki1 antibody, cdkn1a antibody, KRAS antibody
- Molecular Weight
- 21 kDa
- Gene ID
- 1026
- UniProt
- P38936
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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