beta Actin antibody (C-Term)

Catalog No. ABIN6285652

Product Details anti-beta Actin Antibody

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Monkey, Dog, Hamster, Insect

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Beta actin is a protein encoded by the ACTB gene which is approximately 41,7 kDa. Beta actin is localised to the cytoplasm. It is involved in pathways such as blood-brain barrier and immune cell transmigration, regulation of actin cytoskeleton by Rho GTPases and development Slit-Robo signalling. This protein falls under the actin family. There are 3 main groups of actin isoforms in vertebrates namely, alpha, beta and gamma actins. Beta actin is a highly conserved proteins that is involved in various types of internal cell motility and are the major constituent of microfilaments. Beta actin is ubiquitously expressed in all eukaryotic cells. Mutations in the ACTB gene result in a form of juvenile-onset dystonia, which is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. Baraitser-Winter syndrome 1 is another disease that can occur due to mutations. This is rare developmental disorder that causes postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. STJ96930 was developed from clone 60. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody binds endogenous beta actin.

Specificity: The antibody detects endogenous β-actin protein.

Characteristics: Mouse monoclonal to β-actin.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide

Clone: 5B7

Isotype: IgG1

Application Details

Application Notes: WB 1:5000
IHC 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta-actin (ACTB Products)

Synonyms: BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody

Gene ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction